Impact of nutrient supply on growth and synthesis of metabolites of in vitro shoot cultures of S. rebaudiana

Stevia rebaudiana plants produce sweet tasting compounds, steviosides, which are 300 times sweeter as sugar and can be used natural sweeteners. Moreover, stevia leaves contain high amount of antioxidants. The use plant compounds components functional nutrition nutraceuticals is extremely relevant today. purpose this study was to investigate the effect increased nitrogen saccharose supply on growth accumulation steviosides anthocyanins well antioxidative activity in fast-growing shoot cultures S. rebaudiana. morphological changes leaves, anthocyanins, antioxidant properties extracts were determined. It shown that during three weeks vitro cultivation shoots MS medium with double concentration nitrogen, biomass by 71.0%, after application – 133%, increasing combination 162.0% compared control. Sucrose stimulated biomass. Anti-oxidative potential 2.4 higher than control, it 2.7 times. Shoots treated contained 50.7 57.8 mg/g-1 respectively. However, led 73.4 1 steviosides. grown culture had 26.0 content 1.7 greater under added supply, 2.3 saccharose. Optimum media developed individually for each increase production valuable secondary metabolites up 3